CYP24A1 Antibody (OAEB01016)

Catalog No: OAEB01016 (Formerly GWB-EV1034)

Size:100UG

OAEB01016 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Human|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB
• Additional Information: Epitope Info: Internal region
  Immunogen: Peptide with sequence C-RKYDIQATDN, from the internal region of the protein sequence according to NP_000773.2; NP_001122387.1.
  Reactivity Info: Tested: Human, Rat; Expected: Human
  Product Info: This antibody is expected to recognize both reported isoforms (NP_000773.2; NP_001122387.1).
• Reconstitution and Storage: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
  Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: CYP24A1
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human: 100%
• Peptide Sequence: C-RKYDIQATDN
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: Antibody detection limit dilution 1:32000.
  Western Blot: Approx 50kDa band observed in Human and Rat Liver lysates (calculated MW of 51.2kDa according to NP_001122387.1). Recommended concentration: 0.1-0.3ug/ml.
• Reference: Shen H, Bielak LF, Ferguson JF, Streeten EA, Yerges-Armstrong LM, Liu J, Post W, O'Connell JR, Hixson JE, Kardia SL, Sun YV, Jhun MA, Wang X, Mehta NN, Li M, Koller DL, Hakonarson H, Keating BJ, Rader DJ, Shuldiner AR, Peyser PA, Reilly MP, Mitchell BD.; Association of the vitamin D metabolism gene CYP24A1 with coronary artery calcification.; Arterioscler Thromb Vasc Biol. 2010 Dec;30(12):2648-54.; PMID: 20847308

Target Info

• Gene Symbol: CYP24A1
• Gene Full Name: cytochrome P450 family 24 subfamily A member 1
• Alias Symbols: 1,25-@dihydroxyvitamin D3 24-hydroxylase;1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial;24-OHase;CP24;CYP24;cytochrome P450 24A1;cytochrome P450, family 24, subfamily A, polypeptide 1;cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase);cytochrome P450-CC24;exo-mitochondrial protein;HCAI;HCINF1;P450-CC24;vitamin D 24-hydroxylase;vitamin D(3) 24-hydroxylase.
• NCBI Gene Id: 1591
• Protein Name: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
• Uniprot ID: Q07973
• Protein Accession #: NP_000773.2; NP_001122387.1
• Molecular Weight: 58.9; 51.2