Catalog No: OPCA00065
Price: $0.00
SKU
OPCA00065
Availability: Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks
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- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for CYP21A2 Recombinant Protein (Human) (OPCA00065) (OPCA00065) |
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Predicted Species Reactivity | Homo sapiens|Human |
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Product Format | Lyophilized 10mM Tris-HCl, 1mM EDTA, 6% Trehalose (pH 8.0) |
Reconstitution and Storage | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Purification | Affinity purified using IMAC |
Purity | Greater than 90% as determined by SDS-PAGE. |
Protein Sequence | Full Length: MLLLGLLLLPLLAGARLLWNWWKLRSLHLPPLAPGFLHLLQPDLPIYLLGLTQKFGPIYRLHLGLQDVVVLNSKRTIEEAMVKKWADFAGRPEPLTYKLVSKNYPDLSLGDYSLLWKAHKKLTRSALLLGIRDSMEPVVEQLTQEFCERMRAQPGTPVAIEEEFSLLTCSIICYLTFGDKIKDDNLMPAYYKCIQEVLKTWSHWSIQIVDVIPFLRFFPNPGLRRLKQAIEKRDHIVEMQLRQHKESLVAGQWRDMMDYMLQGVAQPSMEEGSGQLLEGHVHMAAVDLLIGGTETTANTLSWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATIAEVLRLRPVVPLALPHRTTRPSSISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWPDRFLEPGKNSRALAFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPHCSVILKMQPFQVRLQPRGMGAHSPGQNQ |
Source | E.coli |
Tag | N-terminal 6xHis-tagged |
Reference | Phenotype-genotype correlations of 13 rare CYP21A2 mutations detected in 46 patients affected with 21-hydroxylase deficiency and in one carrier.Tardy V., Menassa R., Sulmont V., Lienhardt-Roussie A., Lecointre C., Brauner R., David M., Morel Y.J. Clin. Endocrinol. Metab. 95:1288-1300(2010) |
Gene Symbol | CYP21A2 |
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Gene Full Name | cytochrome P450 family 21 subfamily A member 2 |
Alias Symbols | 21-OHase;CA21H;CAH1;CPS1;CYP21;CYP21B;Cytochrome P450 21;cytochrome P450 XXI;cytochrome P450, family 21, subfamily A, polypeptide 2;cytochrome P450, subfamily XXIA (steroid 21-hydroxylase, congenital adrenal hyperplasia), polypeptide 2;Cytochrome P-450c21;Cytochrome P450-C21;cytochrome P450-C21B;P450c21B;steroid 21 hydroxylase;steroid 21-hydroxylase;steroid 21-monooxygenase. |
NCBI Gene Id | 1589 |
Protein Name | Steroid 21-hydroxylase |
Description of Target | A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). |
Uniprot ID | P08686 |
Protein Size (# AA) | Recombinant |
Molecular Weight | 60 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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