CYP21 ELISA Kit (Mouse) (OKEH04241)

Catalog No: OKEH04241

Size:96 Wells

Mouse CYP21 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Cyp21 ELISA Kit (Mouse) (OKEH04241) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cyp21 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cyp21 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cyp21 captured in the well.
• ELISA Kit Range: 0.156 - 10ng/ml
• ELISA Kit Recovery: <= 6.3%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.3% (n = 20)
  Mean Inter-assay CV%: <= 8.2% (n = 20)
• ELISA Kit Component:
  • Cyp21 Microplate: 96 Wells (12 x 8 Well Strips)
  • Cyp21 Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Cyp21 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.09 ng/mL
• Specificity: Natural and recombinant Mouse Steroid 21-hydroxylase
• Publications:

  Interleukin 6 reduces allopregnanolone synthesis in the brain and contributes to age-related cognitive decline in mice. J Lipid Res. 61, 1308-1319 (2020). 32669383

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CYP21
• Gene Full Name: cytochrome P450, family 21, subfamily a, polypeptide 1
• Alias Symbols: 21O, 21-O, 21OH, Cyp2, 21-OH, 21OHA, 21OHB, Cyp21, Oh21-, Cyp21B, Oh21-1, Oh21-2, CYP21OH-A, Cyp21-ps1, Cyp21a2ps, Cyp21a2-ps
• NCBI Gene Id: 13079
• Protein Name: steroid 21-hydroxylase
• Description of Target: Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.
• Uniprot ID: P03940
• Protein Accession #: NP_034125.2
• Nucleotide Accession #: NM_009995.2
• Protein Size (# AA): 487
• Molecular Weight: 55 kDa