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Catalog No: OKEH04241
Price: $525.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Cyp21 ELISA Kit (Mouse) (OKEH04241) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cyp21 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cyp21 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cyp21 captured in the well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit Recovery<= 6.3%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.3% (n = 20)
Mean Inter-assay CV%: <= 8.2% (n = 20)
ELISA Kit Component
Cyp21 Microplate96 Wells (12 x 8 Well Strips)
Cyp21 Standard2 x 2 ng
Sample Diluent1 x 20 mL
Cyp21 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.09 ng/mL
SpecificityNatural and recombinant Mouse Steroid 21-hydroxylase

Interleukin 6 reduces allopregnanolone synthesis in the brain and contributes to age-related cognitive decline in mice. J Lipid Res. 61, 1308-1319 (2020). 32669383

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCyp21a1
Gene Full Namecytochrome P450, family 21, subfamily a, polypeptide 1
Alias Symbols21-hydroxylase;21O;21-O;21OH;21-OH;21OHA;21-OHase;21OHB;Cyp2;Cyp21;Cyp21a2ps;Cyp21a2-ps;Cyp21B;CYP21OH-A;Cyp21-ps1;cytochrome P-450;cytochrome P450 21;cytochrome P450 hydroxylase A;cytochrome P450 XXI;cytochrome P450, 21, pseudogene;cytochrome P450, 21, steroid 21 hydroxylase;cytochrome P450, family 21, subfamily a, polypeptide 2, pseudogene;cytochrome P-450c21;cytochrome P450-C21;Oh21-;Oh21-1;Oh21-2;steroid 21-hydroxylase.
NCBI Gene Id13079
Protein NameP03940
Description of TargetSteroid 21-hydroxylase
Uniprot ID
Protein Accession #NP_034125.2
Nucleotide Accession #NM_009995.2
Protein Size (# AA)487
Molecular Weight55 kDa
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