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Catalog No: OCOA00031
Size:20UG
Price: $179.00
SKU
OCOA00031
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CYP1B1 Protein Lysate (OCOA00031)

Datasheets/ManualsPrintable datasheet for CYP1B1 Protein Lysate (OCOA00031)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionCYP1B1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolCYP1B1
Gene Full Namecytochrome P450 family 1 subfamily B member 1
Alias Symbolsaryl hydrocarbon hydroxylase;ASGD6;CP1B;CYPIB1;cytochrome P450 1B1;cytochrome P450, family 1, subfamily B, polypeptide 1;cytochrome P450, subfamily I (dioxin-inducible), polypeptide 1 (glaucoma 3, primary infantile);dioxin-inducible cytochrome p450;flavoprotein-linked monooxygenase;GLC3A;hydroperoxy icosatetraenoate dehydratase;microsomal monooxygenase;P4501B1;xenobiotic monooxygenase.
NCBI Gene Id1545
Protein NameNM_000104, NP_000095, Q16678
Description of TargetCytochrome P450 1B1, cytochrome P450 1B1 [Homo sapiens], Homo sapiens cytochrome P450 family 1 subfamily B member 1 (CYP1B1), mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q16678
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_000095
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_000104
Molecular Weight60.8 kDa
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