CYP1B1 ELISA Kit (Rat) (OKEH03582)

Catalog No: OKEH03582

Size:96T

Rat CYP1B1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CYP1B1 ELISA Kit (Rat) (OKEH03582) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Cyp1b1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Cyp1b1 and incubated. The Cyp1b1 found in the sample or standards competes with the biotinylated Cyp1b1 for limited binding sites on the immobilized anti-human Cyp1b1 antibody. Excess unbound biotinylated Cyp1b1 and sample or standard Cyp1b1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Cyp1b1 captured in the well and inversely proportional to the amount of Cyp1b1 which was contained in the sample or standard.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Component:
  • Anti-Cyp1b1 Microplate: 96 Wells (12 x 8 Well strips)
  • Cyp1b1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Cyp1b1-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.312 ng/mL
• Specificity: Natural and recombinant Rat Cytochrome P450 1B1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cyp1b1
• Gene Full Name: cytochrome P450, family 1, subfamily b, polypeptide 1
• Alias Symbols: CYPIB1;cytochrome P450 1B1;cytochrome P450, subfamily 1B, polypeptide 1;cytochrome P450RAP;hydroperoxy icosatetraenoate dehydratase;P450RAP.
• NCBI Gene Id: 25426
• Protein Name: Q64678
• Description of Target: Cytochrome P450 1B1
• Uniprot ID: https://www.uniprot.org/uniprot/Q64678
• Protein Accession #: NP_037072.1
• Nucleotide Accession #: NM_012940.2
• Protein Size (# AA): 543
• Molecular Weight: 61 kDa