CYP1B1 ELISA Kit (Human) (OKEH06933)

Catalog No: OKEH06933

Size:96T

Human CYP1B1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology CYP1B1 ELISA Kit (Human) (OKEH06933) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-CYP1B1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CYP1B1 and incubated. The CYP1B1 found in the sample or standards competes with the biotinylated CYP1B1 for limited binding sites on the immobilized anti-CYP1B1 antibody. Excess unbound biotinylated CYP1B1 and sample or standard CYP1B1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CYP1B1 captured in the well and inversely proportional to the amount of CYP1B1 which was contained in the sample or standard.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into matrix = 109%
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
  Intra-Assay CV <7.8%
  Inter-Assay CV <8.9%
• ELISA Kit Component:
  • CYP1B1 Microplate: 96 Wells (12 x 8 Well strips)
  • CYP1B1 Lyophilized Standard: 2 x 50 ng
  • 100X CYP1B1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.407 ng/mL
• Specificity: Natural and recombinant Human Cytochrome P450 1B1
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: CYP1B1
• Gene Full Name: cytochrome P450 family 1 subfamily B member 1
• Alias Symbols: aryl hydrocarbon hydroxylase;ASGD6;CP1B;CYPIB1;cytochrome P450 1B1;cytochrome P450, family 1, subfamily B, polypeptide 1;cytochrome P450, subfamily I (dioxin-inducible), polypeptide 1 (glaucoma 3, primary infantile);dioxin-inducible cytochrome p450;flavoprotein-linked monooxygenase;GLC3A;hydroperoxy icosatetraenoate dehydratase;microsomal monooxygenase;P4501B1;xenobiotic monooxygenase.
• NCBI Gene Id: 1545
• Protein Name: Q16678
• Description of Target: Cytochrome P450 1B1
• Uniprot ID: https://www.uniprot.org/uniprot/Q16678
• Protein Accession #: NP_000095
• Nucleotide Accession #: NM_000104