CYP1B1 ELISA Kit (Human) (OKCD02482)

Catalog No: OKCD02482

Size:96WELLS

Human CYP1B1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 1B1 (CYP1B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 1B1 (CYP1B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 1B1 (CYP1B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome P450 1B1 (CYP1B1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 1B1 (CYP1B1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 1B1 (CYP1B1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CYP1B1 Microplate: 96 Wells (12 x 8 Well strips)
  • CYP1B1 Lyophilized Standard: 2
  • 100X Biotinylated CYP1B1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.54 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 1B1 (CYP1B1). No significant cross-reactivity or interference between Cytochrome P450 1B1 (CYP1B1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CYP1B1
• Gene Full Name: cytochrome P450 family 1 subfamily B member 1
• Alias Symbols: aryl hydrocarbon hydroxylase;ASGD6;CP1B;CYPIB1;cytochrome P450 1B1;cytochrome P450, family 1, subfamily B, polypeptide 1;cytochrome P450, subfamily I (dioxin-inducible), polypeptide 1 (glaucoma 3, primary infantile);dioxin-inducible cytochrome p450;flavoprotein-linked monooxygenase;GLC3A;hydroperoxy icosatetraenoate dehydratase;microsomal monooxygenase;P4501B1;xenobiotic monooxygenase.
• NCBI Gene Id: 1545
• Protein Name: Cytochrome P450 1B1
• Description of Target: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, retinoid and xenobiotics. Preferentially oxidizes 17beta-estradiol to the carcinogenic 4-hydroxy derivative, and a variety of procarcinogenic compounds to their activated forms, including polycyclic aromatic hydrocarbons. Promotes angiogenesis by removing cellular oxygenation products, thereby decreasing oxidative stress, release of antiangiogenic factor THBS2, then allowing endothelial cells migration, cell adhesion and capillary morphogenesis. These changes are concommitant with the endothelial nitric oxide synthase activity and nitric oxide synthesis. Plays an important role in the regulation of perivascular cell proliferation, migration, and survival through modulation of the intracellular oxidative state and NF-kappa-B expression and/or activity, during angiogenesis. Contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression.
• Uniprot ID: Q16678
• Protein Accession #: NP_000095.2
• Nucleotide Accession #: NM_000104.3
• Protein Size (# AA): 543