CYP1B1 Antibody (OABB02029)

Catalog No: OABB02029

Size:100UG

Anti-CYP1B1 Picoband antibody, OABB02029, Western blotting
All lanes: Anti CYP1B1 (OABB02029) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 61KD
Observed bind size: 61KD

Anti-CYP1B1 Picoband antibody, OABB02029, IHC(P)
IHC(P): Human Liver Cancer Tissue

Anti-CYP1B1 Picoband antibody, OABB02029, IHC(P)
IHC(P): Mouse Kidney Tissue

Anti-CYP1B1 Picoband antibody, OABB02029, IHC(P)
IHC(P): Rat Kidney Tissue

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Flow cytometry|Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  Background: Cytochrome P450 1B1 is an enzyme that in humans is encoded by the CYP1B1 gene. CYP1B1 belongs to the the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: E.coli-derived human CYP1B1 recombinant protein (Position: R255-L480). Human CYP1B1 shares 85.4% and 84.5% amino acid (aa) sequence identity with mouse and rat CYP1B1, respectively.
• Purification: Affinity Purified
• Peptide Sequence: Synthetic peptide located within the following region: RTVFREFEQLNRNFSNFILDKFLRHCESLRPGAAPRDMMDAFILSAEKKAAGDSHGGGARLDLENVPATITDIFGASQDTLSTALQWLLLLFTRYPDVQTRVQAELDQVVGRDRLPCMGDQPNLPYVLAFLYEAMRFSSFVPVTIPHATTANTSVLGYHIPKDTVVFVNQWSVNHDPLKWPNPENFDPARFLDKDGLINKDLTSRVMIFSVGKRRCIGEELSKMQL
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Mouse, Rat: By Heat
  Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Human
  Immunocytochemistry: 0.5-1 ug/ml: Human
  Flow Cytometry: 1-3 ug/1x10^6 cells: Human
• Reference: 1. "Entrez Gene: cytochrome P450".
  2. Bailey LR, Roodi N, Dupont WD, Parl FF (1998). "Association of cytochrome P450 1B1 (CYP1B1) polymorphism with steroid receptor status in breast cancer.". Cancer Res. 58 (22): 5038–41.
  3. Michels-Rautenstrauss KG, Mardin CY, Zenker M et al. (2002). "Primary congenital glaucoma: three case reports on novel mutations and combinations of mutations in the GLC3A (CYP1B1) gene.". J. Glaucoma 10 (4): 354–7.
  
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Cytochrome P450 1B1(CYP1B1) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human;Mouse;Rat.

Target Info

• Gene Symbol: CYP1B1
• Gene Full Name: cytochrome P450 family 1 subfamily B member 1
• Alias Symbols: aryl hydrocarbon hydroxylase;ASGD6;CP1B;CYPIB1;cytochrome P450 1B1;cytochrome P450, family 1, subfamily B, polypeptide 1;cytochrome P450, subfamily I (dioxin-inducible), polypeptide 1 (glaucoma 3, primary infantile);dioxin-inducible cytochrome p450;flavoprotein-linked monooxygenase;GLC3A;hydroperoxy icosatetraenoate dehydratase;microsomal monooxygenase;P4501B1;xenobiotic monooxygenase.
• NCBI Gene Id: 1545
• Protein Name: Cytochrome P450 1B1
• Description of Target: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, retinoid and xenobiotics. Preferentially oxidizes 17beta-estradiol to the carcinogenic 4-hydroxy derivative, and a variety of procarcinogenic compounds to their activated forms, including polycyclic aromatic hydrocarbons. Promotes angiogenesis by removing cellular oxygenation products, thereby decreasing oxidative stress, release of antiangiogenic factor THBS2, then allowing endothelial cells migration, cell adhesion and capillary morphogenesis. These changes are concommitant with the endothelial nitric oxide synthase activity and nitric oxide synthesis. Plays an important role in the regulation of perivascular cell proliferation, migration, and survival through modulation of the intracellular oxidative state and NF-kappa-B expression and/or activity, during angiogenesis. Contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression.
• Uniprot ID: Q16678
• Molecular Weight: 60846 MW