CYP1A1 ELISA Kit (Mouse) (OKEH05746)

Catalog No: OKEH05746

Size:96T

Mouse CYP1A1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Cyp1a1 ELISA Kit (Mouse) (OKEH05746) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cyp1a1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Cyp1a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cyp1a1 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 82%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <7.0%, n = 20
  Mean Inter-Assay Precision - <9.4%, n = 20
• ELISA Kit Component:
  • CYP1A1 Microplate: 96 Wells (12 x 8 Well strips)
  • CYP1A1 Lyophilized Standard: 2
  • 100X Biotinylated CYP1A1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.156 ng/mL
• Specificity: Natural and recombinant Mouse Cytochrome P450 1A1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cyp1a1
• Gene Full Name: cytochrome P450, family 1, subfamily a, polypeptide 1
• Alias Symbols: AHH;AHRR;CP11;CYPIA1;cytochrome P450 1A1;cytochrome P450 form 6;cytochrome P450 subfamily I, polypeptide 1;cytochrome P450, 1a1, aromatic compound inducible;cytochrome P450-C;cytochrome P450-P1;hydroperoxy icosatetraenoate dehydratase;P450-;P450-1.
• NCBI Gene Id: 13076
• Protein Name: P00184
• Description of Target: Cytochrome P450 1A1
• Uniprot ID: https://www.uniprot.org/uniprot/P00184
• Protein Accession #: NP_001129531.1
• Nucleotide Accession #: NM_001136059.2
• Protein Size (# AA): 524