Catalog No: OKCD08083
Size:96 Wells
Price: $800.00
SKU
OKCD08083
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CYP19A1 ELISA Kit (Human) (OKCD08083) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYP19A1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYP19A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CYP19A1 captured in the well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CYP19A1. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CYP19A1 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
CYP19A1 Microplate96 Wells (12 x 8 Well strips)
CYP19A1 Lyophilized Standard2
100X CYP19A1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.059ng/mL
Gene SymbolCYP19A1
Gene Full Namecytochrome P450 family 19 subfamily A member 1
Alias SymbolsARO, ARO1, CPV1, CYAR, CYP19, CYPXIX, P-450AROM
NCBI Gene Id1588
Protein NameAromatase
Description of TargetThis gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities.
Swissprot IdP11511
Protein Accession #NP_000094.2
Nucleotide Accession #NM_000103.3
Tissue ToolFind tissues and cell lines supported by RNA-seq analysis to express CYP19A1.
RNA SeqFind tissues and cell lines supported by DNA array analysis to express CYP19A1.
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