Catalog No: OKCD02864
Size:96WELLS
Price: $700.00
SKU
OKCD02864
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Datasheets/ManualsPrintable datasheet for CYP17A1 ELISA Kit (Mouse) (OKCD02864)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
92-99%78-93%95-103%95-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 17A1 (CYP17A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 17A1 (CYP17A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 17A1 (CYP17A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytochrome P450 17A1 (CYP17A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
79-10184
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 17A1 (CYP17A1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 17A1 (CYP17A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CYP17A1 Microplate96 Wells (12 x 8 Well strips)
CYP17A1 Lyophilized Standard2
100X Biotinylated CYP17A1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.58 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of 17-Alpha-Hydroxylase (S17aH). No significant cross-reactivity or interference between 17-Alpha-Hydroxylase (S17aH) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCyp17a1
Gene Full Namecytochrome P450, family 17, subfamily a, polypeptide 1
Alias Symbols17-alpha-hydroxyprogesterone aldolase;Cyp1;Cyp17;CYPXVII;cytochrome P450 17A1;cytochrome P450, 17;cytochrome P450, 17a1;cytochrome P450c17;cytochrome P450-C17;p450c1;p450c17;steroid 17-alpha hydroxylase;steroid 17-alpha-hydroxylase/17,20 lyase;steroid 17-alpha-monooxygenase.
NCBI Gene Id13074
Protein NameSteroid 17-alpha-hydroxylase/17,20 lyase
Description of TargetConversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.
Uniprot IDP27786
Protein Accession #NP_031835.3
Nucleotide Accession #NM_007809.3
Protein Size (# AA)507
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