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CYBRD1 Antibody (OAEB00128)

100 ug
$349.00
In Stock
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Predicted Species Reactivity:
Cow, Dog, Goat, Guinea Pig, Horse, Human, Pig
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Clonality:
Polyclonal
Host:
Goat
Application:
IHC
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Gene Symbol:
CYBRD1
NCBI Gene Id:
79901
Protein Name:
Cytochrome b reductase 1
Swissprot Id:
Q53TN4
Protein Accession #:
NP_079119.3
Alias Symbols:
CYBRD1, DCYTB, FLJ23462, cytochrome b reductase 1, duodenal cytochrome b, FRRS3, ferric-chelate reductase 3
Replacement Item:
This antibody may replace item sc-161022 from Santa Cruz Biotechnology.
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CYBRD1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CYBRD1.
Predicted Homology Based on Immunogen Sequence:
Cow: 85%; Dog: 78%; Goat: 85%; Guinea Pig: 78%; Horse: 85%; Human: 100%; Pig: 92%
Complete computational species homology data:
CYBRD1 Antibody (OAEB00128)
Peptide Sequence:
CRNLALDEAGQRSTM
Datasheets/Manuals:
Printable datasheet for anti-CYBRD1 antibody - OAEB00128
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:16000.
Western Blot: Experiments gave bands at approx 35kDa and a 26+28kDa doublet in human colon lysate after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 31.6kDa according to NP_079119. The detected bands were all successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Target Reference:
* Atanasova BD, Li AC, Bjarnason I, Tzatchev KN, Simpson RJ.; Duodenal ascorbate and ferric reductase in human iron deficiency.; Am J Clin Nutr. 2005 Jan;81(1):130-3.; Pubmed 15640471

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