CYBA Antibody (OASA02663)

Catalog No: OASA02663 (Formerly GWB-82309D)

Size:100UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Phosphate buffered saline.
• Clonality: Monoclonal
• Clone: CS9
• Isotype: IgG1
• Host: Mouse
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry-Frozen|Immunoprecipitation|Western blot
• Additional Information: Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the 3PU1 myeloma cell line.
  Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
  Approx Protein Conc: IgG concentration 1.0mg/ml
  Buffer Solutions: Phosphate buffered saline
• Reconstitution and Storage: -20°C
• Immunogen: Cytochrome B245 solubilised in octyl-beta-gluycopyranoside.
• Purification: Protein G Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Specificity: Mouse anti Human Cytochrome B245 Light Chain antibody, clone CS9 recognizes the light chain of cytochrome B245, also known as p22phox, a subunit of the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase). NADPH oxidase is a membrane-bound enzyme complex in neutrophils that produces the superoxide anion (O2-) by transferring single electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen. Superoxide is used for the destruction of pathogens. Individuals with defects in NADPH oxidase suffer from chronic granulomatous disease, characterised by severe recurrent infections.
  The light chain (p22phox), along with the heavy chain (gp91phox) form cytochrome B245 (also known as flavocytochome b, or Cyt b), which is always membrane-resident. Upon the appropriate stimulus, other components of NADPH oxidase (p47phox, p40phox, p67phox and Rac2) rapidly translocate from the cytosol to the cell membrane to form the complete enzyme complex. Cytochrome B245 appears to serve as a scaffold for the assembly of the other cytosolic units of the oxidase and to provide a transmembrane pathway for electrons.
  The core region of the CS9 epitope is amino acids 165 - 169 (KKPSE), a cytosolic epitope on p22phox not accessible on intact neutrophils. Mouse anti Human Cytochrome B245 Light Chain antibody, clone CS9 also has an inhibitory effect on NADPH oxidase activation.
• Application Info: Flow Cytometry(*): 1/10 - 1/100
  (*) Membrane permeabilisation is required for this application.
• Application Data: Application #1: Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain . Permeabilised with Leucoperm
  Application #2: Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain:Alexa Fluor ?488 . Permeabilised with Leucoperm
  Application #3: Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain:Alexa Fluor ?647 . Permeabilised with Leucoperm
  Application #4: Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Light Chain:FITC . Permeabilised with Leucoperm
• Reference: 1. Taylor, R. M. et al. (2004) Site-specific inhibitors of NADPH oxidase activity and structural probes of flavocytochrome b: characterization of six monoclonal antibodies to the p22phox subunit. J. Immunol. 173: 7349 - 7357
  

Target Info

• Gene Symbol: CYBA
• Alias Symbols: CGD4, p22-PHOX
• NCBI Gene Id: 1535
• Protein Name: Cytochrome b-245 light chain
• Description of Target: MOUSE ANTI HUMAN CYTOCHROME B245 LIGHT CHAIN
  
• Uniprot ID: P13498
• Protein Accession #: NP_000092.2
• Protein Size (# AA): 195