CXCR4 ELISA Kit (Human) : 96 Wells (OKEH01535)

Catalog No: OKEH01535

Size:96 Wells

Human CXCR4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CXCR4 ELISA Kit (Human) (OKEH01535) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXCR4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXCR4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CXCR4 captured in well.
• ELISA Kit Range: 0.156 - 10ng/mL
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.6% (n = 20)
  Mean Inter-assay CV%: < 8.7% (n = 20)
• ELISA Kit Component:
  • CXCR4 Microplate: 96 Wells (12 x 8 Well strips)
  • CXCR4 Lyophilized Standard: 2
  • 100X Biotinylated CXCR4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.052 ng/mL
• Specificity: Natural and recombinant Human C-X-C chemokine receptor type 4
• Publications:

  Protective Effect of β-Glucogallin on Damaged Cataract Against Methylglyoxal Induced Oxidative Stress in Cultured Lens Epithelial Cells. Med Sci Monit. 25, 9310-9318 (2019). 31811113

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CXCR4
• Gene Full Name: C-X-C motif chemokine receptor 4
• Alias Symbols: FB22, HM89, LAP3, LCR1, NPYR, WHIM, CD184, LAP-3, LESTR, NPY3R, NPYRL, WHIMS, HSY3RR, NPYY3R, D2S201E
• NCBI Gene Id: 7852
• Protein Name: C-X-C chemokine receptor type 4
• Description of Target: This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. 
• Uniprot ID: P61073
• Protein Accession #: NP_001008540.1
• Nucleotide Accession #: NM_001008540.1
• Protein Size (# AA): 352
• Molecular Weight: 40 kDa