Catalog No: OKEH01535
Price: $650.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH01535
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CXCR4 ELISA Kit (Human) (OKEH01535) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXCR4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXCR4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CXCR4 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.6% (n = 20)
Mean Inter-assay CV%: < 8.7% (n = 20)
ELISA Kit Component
CXCR4 Microplate96 Wells (12 x 8 Well strips)
CXCR4 Lyophilized Standard2
100X Biotinylated CXCR4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.052 ng/mL
SpecificityNatural and recombinant Human C-X-C chemokine receptor type 4

Protective Effect of β-Glucogallin on Damaged Cataract Against Methylglyoxal Induced Oxidative Stress in Cultured Lens Epithelial Cells. Med Sci Monit. 25, 9310-9318 (2019). 31811113

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCXCR4
Gene Full NameC-X-C motif chemokine receptor 4
Alias SymbolsCD184;CD184 antigen;chemokine (C-X-C motif) receptor 4;C-X-C chemokine receptor type 4;D2S201E;FB22;fusin;HM89;HSY3RR;LAP3;LAP-3;LCR1;LESTR;leukocyte-derived seven transmembrane domain receptor;lipopolysaccharide-associated protein 3;LPS-associated protein 3;neuropeptide Y receptor Y3;neuropeptide Y3 receptor;NPY3R;NPYR;NPYRL;NPYY3R;SDF-1 receptor;seven transmembrane helix receptor;seven-transmembrane-segment receptor, spleen;stromal cell-derived factor 1 receptor;WHIM;WHIMS;WHIMS1.
NCBI Gene Id7852
Protein NameP61073
Description of TargetC-X-C chemokine receptor type 4
Uniprot IDhttps://www.uniprot.org/uniprot/P61073
Protein Accession #NP_001008540.1
Nucleotide Accession #NM_001008540.1
Protein Size (# AA)352
Molecular Weight40 kDa
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