CXCR4 ELISA Kit (Dog) (OKEH08489)

Catalog No: OKEH08489

Size:96T

Dog CXCR4 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Canis lupus, Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology CXCR4 ELISA Kit (Dog) (OKEH08489) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXCR4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXCR4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CXCR4 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.1% (n = 20)
  Mean Inter-assay CV%: <=7.2% (n = 20)
• ELISA Kit Component:
  • CXCR4 Microplate: 96 Wells (12 x 8 Well strips)
  • CXCR4 Lyophilized Standard: 2
  • 100X Biotinylated CXCR4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival.
  Subcellular Location: Cell membrane Multi-pass membrane protein In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated.
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.083ng/mL
• Specificity: Natural and recombinant Dog C-X-C chemokine receptor type 4
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CXCR4
• Gene Full Name: C-X-C motif chemokine receptor 4
• Alias Symbols: chemokine (C-X-C motif) receptor 4;C-X-C chemokine receptor type 4;CXCR-4;CXC-R4.
• NCBI Gene Id: 483900
• Protein Name: Q3LSL6
• Description of Target: C-X-C chemokine receptor type 4
• Uniprot ID: https://www.uniprot.org/uniprot/Q3LSL6
• Protein Accession #: NP_001041491.1
• Nucleotide Accession #: NM_001048026.1