CXCR3 Antibody - middle region (OAAB00176)

Catalog No: OAAB00176

Size:400ul

CXCR3 Antibody (Center) western blot analysis in K562 cell lysates (35ug/lane).This demonstrates the CXCR3 antibody detected the CXCR3 protein (arrow).

CXCR3 is strongly supported by BioGPS gene expression data to be expressed in K562

CXCR3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CXCR3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

CXCR3 Antibody (Center) (OAAB00176) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, FC
• Additional Information: Function: Isoform 1: Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway (PubMed:12782716). Binds to CCL21. Probably promotes cell chemotaxis response. Isoform 3: Mediates the activity of CXCL11.
  Cellular Location: Isoform 1: Cell membrane; Multi-pass membrane protein.
  Tissue Location: Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. Isoform 2 is expressed in endothelial cells. Expressed in T-cells (at protein level).
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This CXCR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-167 amino acids of human CXCR3.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  CXCR3 is strongly supported by BioGPS gene expression data to be expressed in K562

• Intended Use: CXCR3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50

Target Info

• Gene Symbol: CXCR3
• Alias Symbols: GPR9, MigR, CD182, CD183, Mig-R, CKR-L2, CMKAR3, IP10-R
• NCBI Gene Id: 2833
• Protein Name: C-X-C chemokine receptor type 3
• Description of Target: This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed IP10 (interferon-g-inducible 10 kDa protein), Mig (monokine induced by interferon-g) and I-TAC (interferon-inducible T cell a-chemoattractant). IP10, Mig and I-TAC belong to the structural subfamily of CXC chemokines, in which a single amino acid residue separates the first two of four highly conserved Cys residues. Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Inhibition by Bordetella pertussis toxin suggests that heterotrimeric G protein of the Gi-subclass couple to this protein. Signal transduction has not been further analyzed but may include the same enzymes that were identified in the signaling cascade induced by other chemokine receptors. As a consequence of chemokine-induced cellular desensitization (phosphorylation-dependent receptor internalization), cellular responses are typically rapid and short in duration. Cellular responsiveness is restored after dephosphorylation of intracellular receptors and subsequent recycling to the cell surface. This gene is prominently expressed in in vitro cultured effector/memory T cells, and in T cells present in many types of inflamed tissues. In addition, IP10, Mig and I-TAC are commonly produced by local cells in inflammatory lesion, suggesting that this gene and its chemokines participate in the recruitment of inflammatory cells. Therefore, this protein is a target for the development of small molecular weight antagonists, which may be used in the treatment of diverse inflammatory diseases. Multiple transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P49682
• Protein Accession #: NP_001495.1
• Molecular Weight: 40660 Da