CXCR2 Antibody (Phospho-Ser347) (OAAF00235)

Catalog No: OAAF00235 (Formerly GWB-ASB500)

Size:100 ug

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Western blot analysis of extracts from NIH-3T3 cells treated with PMA 125ng/ml 30', using IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of COS7 cells, using IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human IL-8R beta/CDw128 beta around the phosphorylation site of Ser347.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
• Specificity: IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody detects endogenous levels of IL-8R beta/CDw128 beta only when phosphorylated at serine347.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:1000
• Reference: Murphy P.M., Science 253:1280-1283(1991).
  Cerretti D.P., Mol. Immunol. 30:359-367(1993).
  Ahuja S.K., J. Biol. Chem. 269:26381-26389(1994)

Target Info

• Gene Symbol: CXCR2
• Gene Full Name: C-X-C motif chemokine receptor 2
• Alias Symbols: CD182;CDw128b;chemokine (CXC) receptor 2;CMKAR2;C-X-C chemokine receptor type 2;CXCR-2;CXC-R2;CXCR2 gene for IL8 receptor type B;GRO/MGSA receptor;high affinity interleukin-8 receptor B;IL-8 receptor type 2;IL-8R B;IL8R2;IL8RA;IL8RB;interleukin 8 receptor type 2;interleukin 8 receptor, beta;interleukin-8 receptor type B;WHIMS2.
• NCBI Gene Id: 3579
• Protein Name: C-X-C chemokine receptor type 2
• Description of Target: Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
• Uniprot ID: P25025
• Protein Accession #: NP_001161770.1
• Molecular Weight: 40 kDa