Catalog No: OKCD05639
Size:96WELLS
Price: $600.00
SKU
OKCD05639
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Datasheets/ManualsPrintable datasheet for CXCR1 ELISA Kit (Human) (OKCD05639)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 8 Receptor Alpha (IL8Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 8 Receptor Alpha (IL8Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 8 Receptor Alpha (IL8Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 8 Receptor Alpha (IL8Ra) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 8 Receptor Alpha (IL8Ra) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 8 Receptor Alpha (IL8Ra) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CXCR1 Microplate96 Wells (12 x 8 Well strips)
CXCR1 Lyophilized Standard2
100X CXCR1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.054ng/mL
Gene SymbolCXCR1
Gene Full NameC-X-C motif chemokine receptor 1
Alias SymbolsC-C;C-C-CKR-1;CD128;CD181;CDw128a;chemokine (C-X-C motif) receptor 1;CKR-1;CMKAR1;C-X-C chemokine receptor type 1;CXCR-1;CXC-R1;high affinity interleukin-8 receptor A;IL-8 receptor type 1;IL-8R A;IL8R1;IL8RA;IL8RBA;interleukin 8 receptor, alpha;interleukin-8 receptor type 1;interleukin-8 receptor type A.
NCBI Gene Id3577
Protein NameC-X-C chemokine receptor type 1
Description of TargetReceptor to interleukin-8, which is a powerful neutrophils chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity.
Uniprot IDP25024
Protein Accession #NP_000625.1
Nucleotide Accession #NM_000634.2
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