CXCL9 ELISA Kit (Rat) (OKCD07806)

Catalog No: OKCD07806

Size:96WELLS

CXCL9 ELISA Kit (Rat) (OKCD07806) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monokine Induced By Interferon Gamma (MIg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monokine Induced By Interferon Gamma (MIg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monokine Induced By Interferon Gamma (MIg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Monokine Induced By Interferon Gamma (MIg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monokine Induced By Interferon Gamma (MIg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monokine Induced By Interferon Gamma (MIg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CXCL9 Microplate: 96 Wells (12 x 8 Well strips)
  • CXCL9 Lyophilized Standard: 2
  • 100X CXCL9 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 6.1pg/mL

Target Info

• Gene Symbol: Cxcl9
• Gene Full Name: C-X-C motif chemokine ligand 9
• Alias Symbols: chemokine (C-X-C motif) ligand 9;C-X-C motif chemokine 9;Mig;monokine induced by gamma interferon;Scyb9;small inducible cytokine B9.
• NCBI Gene Id: 246759
• Protein Name: C-X-C motif chemokine
• Description of Target: C-X-C motif chemokine
• Uniprot ID: Q8K4B1
• Protein Accession #: NP_663705.1
• Nucleotide Accession #: NM_145672.4