CXCL9 ELISA Kit (Mouse) (OKCD00181)

Catalog No: OKCD00181 (Formerly GWB-KBBWG1)

Size:96WELLS

Mouse Cxcl9 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	95-105%	96-105%	80-104%	90-101%
  EDTA plasma(n=5)	89-98%	80-98%	94-103%	80-93%
  heparin plasma(n=5)	78-95%	93-101%	78-88%	99-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monokine Induced By Interferon Gamma (MIg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monokine Induced By Interferon Gamma (MIg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monokine Induced By Interferon Gamma (MIg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Monokine Induced By Interferon Gamma (MIg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	79-101	78
  EDTA plasma(n=5)	87-94	91
  heparin plasma(n=5)	78-94	87

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monokine Induced By Interferon Gamma (MIg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monokine Induced By Interferon Gamma (MIg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Cxcl9 Microplate: 96 Wells (12 x 8 Well Strips)
  • Cxcl9 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated Cxcl9 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Sensitivity: < 6.7 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Monokine Induced By Interferon Gamma (MIg). No significant cross-reactivity or interference between Monokine Induced By Interferon Gamma (MIg) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Cxcl9
• Gene Full Name: chemokine (C-X-C motif) ligand 9
• Alias Symbols: BB139920;CMK;crg-1;crg-10;C-X-C motif chemokine 9;gamma interferon-induced monokine;Gamma-interferon-induced monokine;M119;Mi;Mig;monokine induced by gamma interferon;monokine induced by interferon-gamma;MuMIG;protein m119;Scyb;Scyb9;small inducible cytokine B subfamily (Cys-X-Cys), member 9;small-inducible cytokine B9.
• NCBI Gene Id: 17329
• Protein Name: C-X-C motif chemokine 9
• Description of Target: May be a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response.
• Uniprot ID: P18340
• Protein Accession #: NP_032625
• Nucleotide Accession #: NM_008599.4
• Protein Size (# AA): 126