CXCL9 ELISA Kit (Mouse) (OKBB00793)

Catalog No: OKBB00793

Size:96 wells

CXCL9 ELISA Kit (Mouse) (OKBB00793) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's mouse CXCL9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL9 has been precoated onto 96-well plates. Standards(E.coli, T22-T126) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CXCL9 amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	86	228	891	79	240	896
  Standard deviation	4.9	13.45	38.31	4.74	17.28	43
  CV(%)	5.7	5.9	4.3	6	7.2	4.8

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Mouse CXCL9 Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Mouse CXCL9 Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Mouse CXCL9 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	86	84	92	82	86	3.74	4.3%
  Sample 2	228	207	217	212	216	7.77	3.5%
  Sample 3	891	891	830	879	872	25.16	2.8%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
  O.D.	0.031	0.074	0.157	0.305	0.587	1.032	1.901	2.369

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse CXCL9
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: T22-T126
• Description: For quantitative detection of mouse CXCL9 in cell culture supernatants, serum and plasma(EDTA).

Target Info

• Gene Symbol: CXCL9
• Gene Full Name: chemokine (C-X-C motif) ligand 9
• Alias Symbols: C-X-C motif chemokine 9, Chemokine (C-X-C motif) ligand 9, CMK, Crg 10, CXCL9, CXCL9_HUMAN, Gamma interferon induced monokine, Gamma-interferon-induced monokine, HuMIG, MIG, Monokine induced by gamma interferon, Monokine induced by interferon gamma, Monokine induced by interferon-gamma, SCYB9, Small inducible cytokine B9, Small inducible cytokine subfamily B member 9, Small-inducible cytokine B9
• NCBI Gene Id: 17329
• Protein Name: C-X-C motif chemokine 9
• Description of Target: Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.
• Uniprot ID: P18340
• Protein Accession #: NP_032625.2
• Nucleotide Accession #: NM_008599.4