CXCL9 ELISA Kit (Bovine) (OKEH08450)

Catalog No: OKEH08450

Size:96T

Bovine CXCL9 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology CXCL9 ELISA Kit (Bovine) (OKEH08450) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXCL9 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXCL9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CXCL9 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 115% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=2.6% (n = 20)
  Mean Inter-assay CV%: <=11.3% (n = 20)
• ELISA Kit Component:
  • CXCL9 Microplate: 96 Wells (12 x 8 Well strips)
  • CXCL9 Lyophilized Standard: 2
  • 100X Biotinylated CXCL9 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
  Subcellular Location: Secreted
  Unigene: Bt.37553
  SMR: A9QWP9
  String: 9913.ENSBTAP00000002257
  KEGG: bta:513990
  Pfam: PF00048
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 19.5pg/mL
• Specificity: Natural and recombinant Bovine C-X-C motif chemokine 9
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CXCL9
• Gene Full Name: C-X-C motif chemokine ligand 9
• Alias Symbols: C-X-C motif chemokine 9;monokine induced by interferon gamma;small-inducible cytokine B9.
• NCBI Gene Id: 513990
• Protein Name: A9QWP9
• Description of Target: C-X-C motif chemokine 9
• Uniprot ID: https://www.uniprot.org/uniprot/A9QWP9
• Protein Accession #: NP_001106643.1
• Nucleotide Accession #: NM_001113172.1