CXCL9 Chemi-Luminescent ELISA Kit (Human) (OKCD03749)

Catalog No: OKCD03749

Size:96WELLS

Human CXCL9 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-104%	93-105%	86-93%	84-93%
  EDTA Plasma (n=5)	82-96%	78-101%	97-104%	90-104%
  Heparin Plasma (n=5)	95-105%	79-93%	98-105%	95-103%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Monokine Induced By Interferon Gamma (MIg). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Monokine Induced By Interferon Gamma (MIg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Monokine Induced By Interferon Gamma (MIg) level in the sample or standard.
• ELISA Kit Range: 13.7-10,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-95	92
  EDTA Plasma (n=5)	88-102	93
  Heparin Plasma (n=5)	83-101	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monokine Induced By Interferon Gamma (MIg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monokine Induced By Interferon Gamma (MIg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CXCL9 Microplate: 96 Wells (12 x 8 Well strips)
  • CXCL9 Lyophilized Standard: 2
  • 100X CXCL9 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.156 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CXCL9
• Gene Full Name: C-X-C motif chemokine ligand 9
• Alias Symbols: chemokine (C-X-C motif) ligand 9;CMK;crg-10;C-X-C motif chemokine 9;gamma-interferon-induced monokine;Humig;MIG;monokine induced by gamma interferon;monokine induced by interferon-gamma;SCYB9;small-inducible cytokine B9.
• NCBI Gene Id: 4283
• Protein Name: C-X-C motif chemokine 9
• Description of Target: Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
• Uniprot ID: Q07325
• Protein Accession #: NP_002407
• Nucleotide Accession #: NM_002416