CXCL8 Chemi-Luminescent ELISA Kit (Human) (OKCD03689)

Catalog No: OKCD03689

Size:96WELLS

Human CXCL8 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	90-98%	82-92%	80-104%	98-105%
  EDTA Plasma (n=5)	86-94%	90-102%	88-104%	88-102%
  Heparin Plasma (n=5)	94-101%	96-105%	79-88%	85-101%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 8 (IL8). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 8 (IL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 8 (IL8) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	90-103	94
  EDTA Plasma (n=5)	95-105	102
  Heparin Plasma (n=5)	79-97	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CXCL8 Microplate: 96 Wells (12 x 8 Well strips)
  • CXCL8 Lyophilized Standard: 2
  • 100X CXCL8 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 1.37 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CXCL8
• Gene Full Name: C-X-C motif chemokine ligand 8
• Alias Symbols: alveolar macrophage chemotactic factor I;beta endothelial cell-derived neutrophil activating peptide;beta-thromboglobulin-like protein;chemokine (C-X-C motif) ligand 8;C-X-C motif chemokine 8;emoctakin;GCP1;GCP-1;granulocyte chemotactic protein 1;IL8;interleukin 8;interleukin-8;LECT;LUCT;lung giant cell carcinoma-derived chemotactic protein;lymphocyte derived neutrophil activating peptide;LYNAP;MDNCF;MONAP;monocyte-derived neutrophil chemotactic factor;monocyte-derived neutrophil-activating peptide;NAF;NAP1;NAP-1;neutrophil-activating peptide 1;Neutrophil-activating protein 1;Protein 3-10C;SCYB8;small inducible cytokine subfamily B, member 8;T-cell chemotactic factor;tumor necrosis factor-induced gene 1.
• NCBI Gene Id: 3576
• Protein Name: Interleukin-8
• Description of Target: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
• Uniprot ID: p10145
• Protein Accession #: NP_000575
• Nucleotide Accession #: NM_000584