CXCL6 ELISA Kit (Human) (OKCD07498)

Catalog No: OKCD07498

Size:96WELLS

CXCL6 ELISA Kit (Human) (OKCD07498) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granulocyte Chemotactic Protein 2 (GCP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Granulocyte Chemotactic Protein 2 (GCP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granulocyte Chemotactic Protein 2 (GCP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Granulocyte Chemotactic Protein 2 (GCP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Granulocyte Chemotactic Protein 2 (GCP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Granulocyte Chemotactic Protein 2 (GCP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CXCL6 Microplate: 96 Wells (12 x 8 Well strips)
  • CXCL6 Lyophilized Standard: 2
  • 100X CXCL6 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 6.5pg/mL

Target Info

• Gene Symbol: CXCL6
• Gene Full Name: C-X-C motif chemokine ligand 6
• Alias Symbols: chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2);chemokine alpha 3;CKA-3;C-X-C motif chemokine 6;GCP2;GCP-2;granulocyte chemotactic protein 2;SCYB6;small inducible cytokine subfamily B (Cys-X-Cys), member 6 (granulocyte chemotactic protein 2);small inducible cytokine subfamily B (Cys-X-Cys), member b;small-inducible cytokine B6.
• NCBI Gene Id: 6372
• Protein Name: C-X-C motif chemokine 6
• Description of Target: Chemotactic for neutrophil granulocytes. Signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).
• Uniprot ID: P80162
• Protein Accession #: NP_002984.1
• Nucleotide Accession #: NM_002993.3