CXCL6 Chemi-Luminescent ELISA Kit (Human) (OKCD03504)

Catalog No: OKCD03504

Size:96WELLS

Human CXCL6 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	91-99%	89-97%	93-102%	91-101%
  EDTA Plasma (n=5)	92-105%	97-105%	79-90%	81-101%
  Heparin Plasma (n=5)	98-105%	90-101%	82-94%	89-96%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Granulocyte Chemotactic Protein 2 (GCP2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Granulocyte Chemotactic Protein 2 (GCP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Granulocyte Chemotactic Protein 2 (GCP2) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	94-102	98
  EDTA Plasma (n=5)	85-95	91
  Heparin Plasma (n=5)	98-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Granulocyte Chemotactic Protein 2 (GCP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Granulocyte Chemotactic Protein 2 (GCP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CXCL6 Microplate: 96 Wells (12 x 8 Well strips)
  • CXCL6 Lyophilized Standard: 2
  • 100X CXCL6 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 31.25 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CXCL6
• Gene Full Name: C-X-C motif chemokine ligand 6
• Alias Symbols: chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2);chemokine alpha 3;CKA-3;C-X-C motif chemokine 6;GCP2;GCP-2;granulocyte chemotactic protein 2;SCYB6;small inducible cytokine subfamily B (Cys-X-Cys), member 6 (granulocyte chemotactic protein 2);small inducible cytokine subfamily B (Cys-X-Cys), member b;small-inducible cytokine B6.
• NCBI Gene Id: 6372
• Protein Name: C-X-C motif chemokine 6
• Description of Target: Chemotactic for neutrophil granulocytes. Signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).
• Uniprot ID: P80162
• Protein Accession #: NP_002984
• Nucleotide Accession #: NM_002993