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Catalog No: OKBB00377
Size:96 Wells
Price: $583.00
SKU
OKBB00377
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's mouse CXCL5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL5 has been precoated onto 96-well plates. Standards(E.coli, V45-A118) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CXCL5 amount of sample captured in plate.
ELISA Kit Range15.6 pg/ml - 1,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)89238496103276513
SD4.5413.318.97.4220.732.8
CV%5.15.63.87.27.56.4
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Mouse ENA-78 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Mouse ENA-78 Standard2 x 10 ng/tube
100X Biotinylated Anti-Mouse ENA-78 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant mouse CXCL5
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: E.coli; Immunogen sequence: V45-A118
DescriptionFor quantitative detection of mouse CXCL5 in cell culture supernatants, serum and plasma(heparin, EDTA, citrate).
Gene SymbolCXCL5
Gene Full Namechemokine (C-X-C motif) ligand 5
Alias SymbolsAMCF II, AMCFII, C X C motif chemokine 5, Chemokine (C X C motif) ligand 5, CXCL 5, ENA 78, ENA78, Epithelial derived neutrophil activating protein 78, GCP 2, LIX, Neutrophil activating peptide ENA 78, Neutrophil activating protein 78, SCYB5, Scyb6, Small inducible cytokine B5, Small inducible cytokine subfamily B (Cys X Cys) member 5 (epithelial derived neutrophil activating peptide 78), Small inducible cytokine subfamily B, member 15
NCBI Gene Id20311
Protein NameC-X-C motif chemokine 5
Description of TargetC-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.
Uniprot IDP50228
Protein Accession #NP_033167.2
Nucleotide Accession #NM_009141.3
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