Catalog No: OKCD03432
Size:96WELLS
Price: $850.00
SKU
OKCD03432
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CXCL5 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03432)

Datasheets/ManualsPrintable datasheet for CXCL5 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03432)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)90-99%85-92%80-92%90-99%
EDTA Plasma (n=5)85-99%92-101%91-101%82-96%
Heparin Plasma (n=5)98-105%88-96%92-99%80-94%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Epithelial Neutrophil Activating Peptide 78 (ENA78). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Epithelial Neutrophil Activating Peptide 78 (ENA78). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Epithelial Neutrophil Activating Peptide 78 (ENA78) level in the sample or standard.
ELISA Kit Range5.49-4,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10194
EDTA Plasma (n=5)90-9894
Heparin Plasma (n=5)80-9283
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epithelial Neutrophil Activating Peptide 78 (ENA78) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epithelial Neutrophil Activating Peptide 78 (ENA78) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Cxcl5 Microplate96 Wells (12 x 8 Well strips)
Cxcl5 Lyophilized Standard2
100X Cxcl5 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity15.63 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCxcl5
Gene Full Namechemokine (C-X-C motif) ligand 5
Alias SymbolsAMCF;AMCF-II;C-X-C motif chemokine 5;Cxcl;Cxcl6;cytokine LIX;ENA-;ENA-78;GCP-;GCP-2;granulocyte chemotactic protein-2;L;LIX;Scyb;Scyb5;Scyb6;small inducible cytokine B subfamily, member 5;small inducible cytokine subfamily B, member 15;small-inducible cytokine B5.
NCBI Gene Id20311
Protein NameC-X-C motif chemokine 5
Description of TargetMay participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2(1-78) and, more potent, GCP-2(9-78) attract neutrophils and are involved in neutrophil activation.
Uniprot IDP50228
Protein Accession #NP_033167
Nucleotide Accession #NM_009141
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