CXCL5 Chemi-Luminescent ELISA Kit (Human) (OKCD03431)

Catalog No: OKCD03431

Size:96WELLS

Human CXCL5 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	85-95%	84-93%	81-95%	78-105%
  EDTA Plasma (n=5)	83-98%	92-101%	91-98%	97-105%
  Heparin Plasma (n=5)	91-103%	95-103%	91-104%	87-97%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Epithelial Neutrophil Activating Peptide 78 (ENA78). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Epithelial Neutrophil Activating Peptide 78 (ENA78). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Epithelial Neutrophil Activating Peptide 78 (ENA78) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	82-96	92
  EDTA Plasma (n=5)	78-103	87
  Heparin Plasma (n=5)	81-98	86

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epithelial Neutrophil Activating Peptide 78 (ENA78) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epithelial Neutrophil Activating Peptide 78 (ENA78) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CXCL5 Microplate: 96 Wells (12 x 8 Well strips)
  • CXCL5 Lyophilized Standard: 2
  • 100X CXCL5 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 15.63 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CXCL5
• Gene Full Name: C-X-C motif chemokine ligand 5
• Alias Symbols: chemokine (C-X-C motif) ligand 5;C-X-C motif chemokine 5;ENA-78;ENA-78(1-78);epithelial-derived neutrophil-activating protein 78;neutrophil-activating peptide ENA-78;neutrophil-activating protein 78;SCYB5;small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78);Small-inducible cytokine B5.
• NCBI Gene Id: 6374
• Protein Name: C-X-C motif chemokine 5
• Description of Target: Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
• Uniprot ID: P42830
• Protein Accession #: NP_002985
• Nucleotide Accession #: NM_002994