Catalog No: OKEH02982
Price: $425.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02982
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CXCL3 ELISA Kit (Mouse) (OKEH02982) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cxcl3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Cxcl3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cxcl3 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit Recovery<= 5.3%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.4% (n = 20)
Mean Inter-assay CV%: <= 7.0% (n = 20)
ELISA Kit Component
Anti-Cxcl3 Microplate96 Wells (12 x 8 Well strips)
Cxcl3 Lyophilized Standard2
100X Biotinylated Cxcl3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity39 pg/mL
SpecificityNatural and recombinant Mouse C-X-C motif chemokine 3

Cancer cell-derived interleukin-33 decoy receptor sST2 enhances orthotopic tumor growth in a murine pancreatic cancer model. e0232230 (2020). 32340025

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCxcl3
Gene Full Namechemokine (C-X-C motif) ligand 3
Alias SymbolsC-X-C motif chemokine 3;Dci;Dcip1;dendritic cell inflammatory protein 1;Gm1960.
NCBI Gene Id330122
Protein NameQ6W5C0
Description of TargetC-X-C motif chemokine 3
Uniprot IDhttps://www.uniprot.org/uniprot/Q6W5C0
Protein Accession #NP_976065.1
Nucleotide Accession #NM_203320.3
Protein Size (# AA)100
Molecular Weight11 kDa
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