CXCL3 ELISA Kit (Mouse) (OKEH02982)

Catalog No: OKEH02982

Size:96 Wells

Mouse CXCL3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CXCL3 ELISA Kit (Mouse) (OKEH02982) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cxcl3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Cxcl3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cxcl3 captured in well.
• ELISA Kit Range: 78 - 5000pg/mL
• ELISA Kit Recovery: <= 5.3%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 5.4% (n = 20)
  Mean Inter-assay CV%: <= 7.0% (n = 20)
• ELISA Kit Component:
  • Anti-Cxcl3 Microplate: 96 Wells (12 x 8 Well strips)
  • Cxcl3 Lyophilized Standard: 2
  • 100X Biotinylated Cxcl3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 39 pg/mL
• Specificity: Natural and recombinant Mouse C-X-C motif chemokine 3
• Publications:

  Cancer cell-derived interleukin-33 decoy receptor sST2 enhances orthotopic tumor growth in a murine pancreatic cancer model. e0232230 (2020). 32340025

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CXCL3
• Gene Full Name: chemokine (C-X-C motif) ligand 3
• Alias Symbols: Dci, Dcip1, Gm1960
• NCBI Gene Id: 330122
• Protein Name: C-X-C motif chemokine 3
• Description of Target: Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion.
• Uniprot ID: Q6W5C0
• Protein Accession #: NP_976065.1
• Nucleotide Accession #: NM_203320.3
• Protein Size (# AA): 100
• Molecular Weight: 11 kDa