Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any help please email info@avivasysbio.com

Catalog No: OKCD07520
Size:96WELLS
Price: $750.00
SKU
OKCD07520
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for CXCL3 ELISA Kit (Human) (OKCD07520)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine (C-X-C Motif) Ligand 3 (CXCL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine (C-X-C Motif) Ligand 3 (CXCL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine (C-X-C Motif) Ligand 3 (CXCL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chemokine (C-X-C Motif) Ligand 3 (CXCL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range7.8-500pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 3 (CXCL3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 3 (CXCL3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CXCL3 Microplate96 Wells (12 x 8 Well strips)
CXCL3 Lyophilized Standard2
100X CXCL3 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 3.1pg/mL
Gene SymbolCXCL3
Gene Full NameC-X-C motif chemokine ligand 3
Alias Symbolschemokine (C-X-C motif) ligand 3;CINC-2b;C-X-C motif chemokine 3;GRO3;GRO3 oncogene;GROg;GRO-gamma;GRO-gamma(1-73);growth-regulated protein gamma;macrophage inflammatory protein 2-beta;melanoma growth stimulatory activity gamma;MGSA gamma;MIP-2b;MIP2B;MIP2-beta;SCYB3.
NCBI Gene Id2921
Protein NameC-X-C motif chemokine 3
Description of TargetLigand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.
Uniprot IDP19876
Protein Accession #NP_002081.2
Nucleotide Accession #NM_002090.2
Write Your Own Review
You're reviewing:CXCL3 ELISA Kit (Human) (OKCD07520)
Your Rating
We found other products you might like!