Cxcl2 ELISA Kit (Mouse) (OKEH04515)

Catalog No: OKEH04515

Size:96T

Mouse Cxcl2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: ELISA-Comp
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Cxcl2 ELISA Kit (Mouse) (OKEH04515) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cxcl2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Cxcl2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cxcl2 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-Cxcl2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Cxcl2 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Cxcl2 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids
• Sensitivity: 0.166 ng/mL
• Specificity: Natural and recombinant Mouse C-X-C motif chemokine 2
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Cxcl2
• Gene Full Name: chemokine (C-X-C motif) ligand 2
• Alias Symbols: CINC;CINC-2a;C-X-C motif chemokine 2;Gr;Gro2;GROb;macrophage inflammatory protein 2;Mgsa;Mgsa-b;Mi;MIP;Mip2;MIP-2;MIP-2a;Scy;Scyb;Scyb2;small inducible cytokine subfamily, member 2.
• NCBI Gene Id: 20310
• Protein Name: P10889
• Description of Target: C-X-C motif chemokine 2
• Uniprot ID: https://www.uniprot.org/uniprot/P10889
• Protein Accession #: NP_033166.1
• Nucleotide Accession #: NM_009140.2
• Protein Size (# AA): 100
• Molecular Weight: 10 KDa