CXCL2 Chemi-Luminescent ELISA Kit (Human) (OKCD03543)

Catalog No: OKCD03543

Size:96WELLS

Human CXCL2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	82-98%	94-103%	81-93%	91-101%
  EDTA Plasma (n=5)	97-105%	86-99%	94-102%	86-102%
  Heparin Plasma (n=5)	99-105%	82-101%	86-93%	93-101%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Chemokine (C-X-C Motif) Ligand 2 (CXCL2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine (C-X-C Motif) Ligand 2 (CXCL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine (C-X-C Motif) Ligand 2 (CXCL2) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	96-103	101
  EDTA Plasma (n=5)	80-93	87
  Heparin Plasma (n=5)	90-98	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 2 (CXCL2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 2 (CXCL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CXCL2 Microplate: 96 Wells (12 x 8 Well strips)
  • CXCL2 Lyophilized Standard: 2
  • 100X CXCL2 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 15.63 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CXCL2
• Gene Full Name: C-X-C motif chemokine ligand 2
• Alias Symbols: chemokine (C-X-C motif) ligand 2;CINC-2a;C-X-C motif chemokine 2;GRO2;GRO2 oncogene;GROb;gro-beta;growth-regulated protein beta;macrophage inflammatory protein 2-alpha;melanoma growth stimulatory activity beta;MGSA beta;MGSA-b;MIP2;MIP-2a;MIP2A;MIP2-alpha;SCYB2.
• NCBI Gene Id: 2920
• Protein Name: C-X-C motif chemokine 2
• Description of Target: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
• Uniprot ID: P19875
• Protein Accession #: NP_002080
• Nucleotide Accession #: NM_002089