CXCL17 ELISA Kit (Rat) (OKCD00185)

Catalog No: OKCD00185 (Formerly GWB-KBBWY8)

Size:96WELLS

Rat Cxcl17 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	79-99%	85-92%	96-104%	79-91%
  EDTA plasma(n=5)	82-101%	86-103%	80-91%	79-99%
  heparin plasma(n=5)	81-103%	78-96%	79-94%	90-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VEGF Co Regulated Chemokine 1 (VCC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VEGF Co Regulated Chemokine 1 (VCC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VEGF Co Regulated Chemokine 1 (VCC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VEGF Co Regulated Chemokine 1 (VCC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	78-104	84
  EDTA plasma(n=5)	79-105	92
  heparin plasma(n=5)	80-97	86

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VEGF Co Regulated Chemokine 1 (VCC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VEGF Co Regulated Chemokine 1 (VCC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Cxcl17 Microplate: 96 Wells (12 x 8 Well Strips)
  • Cxcl17 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated Cxcl17 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 6.1 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of VEGF Co Regulated Chemokine 1 (VCC1). No significant cross-reactivity or interference between VEGF Co Regulated Chemokine 1 (VCC1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Cxcl17
• Gene Full Name: C-X-C motif chemokine ligand 17
• Alias Symbols: chemokine (C-X-C motif) ligand 17;C-X-C motif chemokine 17;RGD1304717;VEGF coregulated chemokine 1;VEGF co-regulated chemokine 1.
• NCBI Gene Id: 308436
• Protein Name: C-X-C motif chemokine ligand 17
• Description of Target: C-X-C motif chemokine ligand 17
• Uniprot ID: D4A875
• Protein Accession #: NP_001100961
• Nucleotide Accession #: NM_001107491.1
• Protein Size (# AA): 119