Catalog No: OKCD00119 (Formerly GWB-KBBUY6)
Size:96 Wells
Price: $675.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
EDTA plasma(n=5)84-101%94-101%99-105%88-99%
heparin plasma(n=5)98-105%87-104%95-103%79-103%
ELISA Kit PrincipleAviva Systems Biology CXCL17 ELISA Kit (Human) (OKCD00119) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXCL17 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXCL17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CXCL17captured in well.
ELISA Kit Range15.6-1,000 pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
EDTA plasma(n=5)80-9487
heparin plasma(n=5)85-9789
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level CXCL17 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CXCL17 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
Anti-CXCL17 Microplate96 Wells (12 x 8 Well Strips)
CXCL17 Lyophilized Standard2 x 1 ng
100X Biotinylated CXCL17 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 5.9 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of VEGF Co Regulated Chemokine 1 (VCC1). No significant cross-reactivity or interference between VEGF Co Regulated Chemokine 1 (VCC1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCXCL17
Gene Full NameC-X-C motif chemokine ligand 17
Alias SymbolsDMC, VCC1, Dcip1, VCC-1, UNQ473
NCBI Gene Id284340
Protein NameVEGF coregulated chemokine 1
Description of TargetPlays a role in angiogenesis and possibly in the development of tumors. May be a housekeeping chemokine regulating recruitment of nonactivated blood monocytes and immature dendritic cells into tissues. May play a role in the innate defense against infections.
Swissprot IdQ6UXB2
Protein Accession #NP_940879
Nucleotide Accession #NM_198477.2
Protein Size (# AA)119
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CXCL17.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CXCL17.
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