Catalog No: OKCD04016
Size:96WELLS
Price: $800.00
SKU
OKCD04016
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CXCL17 Chemi-Luminescent ELISA Kit (Human) (OKCD04016)

Datasheets/ManualsPrintable datasheet for CXCL17 Chemi-Luminescent ELISA Kit (Human) (OKCD04016)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)86-99%88-102%79-92%85-101%
EDTA Plasma (n=5)79-102%86-104%92-105%93-102%
Heparin Plasma (n=5)92-105%81-89%95-102%79-98%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to VEGF Co Regulated Chemokine 1 (VCC1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to VEGF Co Regulated Chemokine 1 (VCC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the VEGF Co Regulated Chemokine 1 (VCC1) level in the sample or standard.
ELISA Kit Range4.12-3,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)95-10399
EDTA Plasma (n=5)78-10397
Heparin Plasma (n=5)78-9488
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VEGF Co Regulated Chemokine 1 (VCC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VEGF Co Regulated Chemokine 1 (VCC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CXCL17 Microplate96 Wells (12 x 8 Well strips)
CXCL17 Lyophilized Standard2
100X CXCL17 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity4.1 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCXCL17
Gene Full NameC-X-C motif chemokine ligand 17
Alias Symbols6-Cys CXCL17;chemokine (C-X-C motif) ligand 17;C-X-C motif chemokine 17;Dcip1;dendritic cell and monocyte chemokine-like protein;DMC;UNQ473;VCC1;VCC-1;VEGF coregulated chemokine 1;VEGF co-regulated chemokine 1.
NCBI Gene Id284340
Protein NameC-X-C motif chemokine 17
Description of TargetChemokine that acts as chemoattractant for monocytes, macrophages and dendritic cells (PubMed:16455961, PubMed:23115081). Plays a role in angiogenesis and possibly in the development of tumors (PubMed:16989774, PubMed:23115081). Acts as an anti-inflammatory in the stomach (PubMed:23115081). May play a role in the innate defense against infections (PubMed:17307946). Activates the C-X-C chemokine receptor GPR35 to induce a rapid and transient rise in the level of intracellular calcium ions (PubMed:25411203).
Uniprot IDQ6UXB2
Protein Accession #NP_940879
Nucleotide Accession #NM_198477
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