CXCL16 ELISA Kit (Rat) (OKCD02368)

Catalog No: OKCD02368

Size:96WELLS

Rat CXCL16 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	80-96%	78-102%	91-98%	85-101%
  EDTA Plasma (n=5)	87-105%	92-101%	87-94%	80-94%
  Heparin Plasma (n=5)	90-104%	93-101%	95-103%	80-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X-C-Motif Ligand 16 (CXCL16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-X-C-Motif Ligand 16 (CXCL16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X-C-Motif Ligand 16 (CXCL16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chemokine C-X-C-Motif Ligand 16 (CXCL16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-89	86
  EDTA Plasma (n=5)	90-97	93
  Heparin Plasma(n=5)	87-98	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-X-C-Motif Ligand 16 (CXCL16) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-X-C-Motif Ligand 16 (CXCL16) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CXCL16 Microplate: 96 Wells (12 x 8 Well strips)
  • CXCL16 Lyophilized Standard: 2
  • 100X Biotinylated CXCL16 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture cell supernatant and other biological fluids
• Sensitivity: 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chemokine C-X-C-Motif Ligand 16 (CXCL16). No significant cross-reactivity or interference between Chemokine C-X-C-Motif Ligand 16 (CXCL16) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cxcl16
• Gene Full Name: C-X-C motif chemokine ligand 16
• Alias Symbols: chemokine (C-X-C motif) ligand 16;C-X-C motif chemokine 16;small-inducible cytokine B16;transmembrane chemokine CXCL16.
• NCBI Gene Id: 497942
• Protein Name: C-X-C motif chemokine 16
• Description of Target: Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. Also acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity).
• Uniprot ID: Q6AXU5
• Protein Accession #: NP_001017478.1
• Nucleotide Accession #: NM_001017478.1
• Protein Size (# AA): 247