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Catalog No: OKCD07522
Size:96WELLS
Price: $750.00
SKU
OKCD07522
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Datasheets/ManualsPrintable datasheet for CXCL14 ELISA Kit (Human) (OKCD07522)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine (C-X-C Motif) Ligand 14 (CXCL14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine (C-X-C Motif) Ligand 14 (CXCL14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine (C-X-C Motif) Ligand 14 (CXCL14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chemokine (C-X-C Motif) Ligand 14 (CXCL14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 14 (CXCL14) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 14 (CXCL14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CXCL14 Microplate96 Wells (12 x 8 Well strips)
CXCL14 Lyophilized Standard2
100X CXCL14 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity< 6.0pg/mL
Gene SymbolCXCL14
Gene Full NameC-X-C motif chemokine ligand 14
Alias SymbolsBMAC;bolekine;BRAK;breast and kidney;chemokine (C-X-C motif) ligand 14;chemokine BRAK;CXC chemokine in breast and kidney;C-X-C motif chemokine 14;KEC;KS1;MIP-2 gamma;MIP-2g;MIP2G;NJAC;SCYB14;small inducible cytokine subfamily B (Cys-X-Cys), member 14 (BRAK);small-inducible cytokine B14;tumor-suppressing chemokine.
NCBI Gene Id9547
Protein NameC-X-C motif chemokine 14
Description of TargetPotent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.
Uniprot IDO95715
Protein Accession #NP_004878.2
Nucleotide Accession #NM_004887.4
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