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Catalog No: OKCD03338
Size:96WELLS
Price: $875.00
SKU
OKCD03338
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CXCL14 Chemi-Luminescent ELISA Kit (Rat) (OKCD03338)

Datasheets/ManualsPrintable datasheet for CXCL14 Chemi-Luminescent ELISA Kit (Rat) (OKCD03338)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)97-104%87-96%97-105%91-99%
EDTA Plasma (n=5)81-99%91-98%93-101%89-97%
Heparin Plasma (n=5)78-104%80-92%97-105%90-98%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Chemokine (C-X-C Motif) Ligand 14 (CXCL14). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine (C-X-C Motif) Ligand 14 (CXCL14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine (C-X-C Motif) Ligand 14 (CXCL14) level in the sample or standard.
ELISA Kit Range13.7-10,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)79-9585
EDTA Plasma (n=5)80-10396
Heparin Plasma (n=5)79-9787
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 14 (CXCL14) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 14 (CXCL14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Cxcl14 Microplate96 Wells (12 x 8 Well strips)
Cxcl14 Lyophilized Standard2
100X Cxcl14 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.156 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCxcl14
Gene Full NameC-X-C motif chemokine ligand 14
Alias SymbolsBRAK;chemokine (C-X-C motif) ligand 14;C-X-C motif chemokine 14.
NCBI Gene Id306748
Protein NameBRAK
Uniprot IDD4A5S7
Protein Accession #NP_001013155
Nucleotide Accession #NM_001013137
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