CXCL14 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03729)

Catalog No: OKCD03729

Size:96WELLS

Mouse CXCL14 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	85-93%	95-102%	83-92%	97-104%
  EDTA Plasma (n=5)	93-101%	84-91%	79-95%	92-101%
  Heparin Plasma (n=5)	79-101%	79-105%	80-97%	93-101%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Chemokine (C-X-C Motif) Ligand 14 (CXCL14). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine (C-X-C Motif) Ligand 14 (CXCL14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine (C-X-C Motif) Ligand 14 (CXCL14) level in the sample or standard.
• ELISA Kit Range: 13.7-10,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	92-101	96
  EDTA Plasma (n=5)	82-90	85
  Heparin Plasma (n=5)	89-103	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 14 (CXCL14) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 14 (CXCL14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Cxcl14 Microplate: 96 Wells (12 x 8 Well strips)
  • Cxcl14 Lyophilized Standard: 2
  • 100X Cxcl14 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.156 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cxcl14
• Gene Full Name: chemokine (C-X-C motif) ligand 14
• Alias Symbols: 1110031L23Rik;1200006I23Rik;AI414372;B-cell and monocyte-activating chemokine;BMAC;bolekine;BR;BRAK;chemokine BRAK;C-X-C motif chemokine 14;Kec;kidney-expressed chemokine CXC;KS1;MIP-;MIP-2g;MIP2gamma;musculus CXC chemokine MIP-2gamma;NJAC;Scyb1;Scyb14;small inducible cytokine subfamily B (Cys-X-Cys), member 14;small-inducible cytokine B14.
• NCBI Gene Id: 57266
• Protein Name: C-X-C motif chemokine 14
• Description of Target: Chemotactic for CESS B-cells and THP-1 monocytes, but not T-cells.
• Uniprot ID: Q9WUQ5
• Protein Accession #: NP_062514
• Nucleotide Accession #: NM_019568