- Predicted Species Reactivity:
- Human
- Reconstitution and Storage:
- Store as indicated in product manual.
- Alias Symbols:
- BMAC, bolekine, BRAK, Chemokine BRAK, C-X-C motif chemokine 14, Kec, KEC, KS1, MGC10687, MIP2G, MIP-2g, MIP-2G, NJAC, PSEC0212, SCYB14, Small-inducible cytokine B14, UNQ240/PRO273
- NCBI Gene Id:
- 9547
- Protein Name:
- C-X-C motif chemokine 14
- Description of Target:
- Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.
- Sample Type:
- Serum, plasma and other biological fluids
- Sensitivity:
- 0.156 ng/mL
- Kit Range:
- 0.156 - 10 ng/mL
- Kit Reproducibility:
- Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CXCL14. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CXCL14 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
- Kit Duration:
- ~ 3.5 Hours
- Kit Principle:
- Aviva Systems Biology CXCL14 Chemi-Luminescent ELISA Kit (Human) (OKCD03339) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXCL14 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXCL14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample CXCL14
- Kit Component:
Component | Amount |
CXCL14 Microplate | 96 Wells (12 x 8 Well strips) |
CXCL14 Lyophilized Standard | 2 |
100X CXCL14 Biotinylated Detector Anitbody | 1 x 120 uL |
100X Avidin-HRP Conjugate | 1 x 120 uL |
Standard Diluent | 1 x 20 mL |
Detector Antibody Diluent | 1 x 12 mL |
Conjugate Diluent | 1 x 12 mL |
30X Wash Buffer | 1 x 20 mL |
100X Luminol Substrate | 1 x 2 mL |
Substrate Diluent | 1 x 20 mL |
- Kit Linearity:
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
Serum (n=5) | 90-99% | 89-104% | 98-105% | 86-93% |
EDTA Plasma (n=5) | 80-101% | 94-103% | 78-102% | 96-103% |
Heparin Plasma (n=5) | 92-99% | 88-96% | 83-99% | 86-94% |
- Kit Recovery:
Matrix | Recovery range (%) | Average(%) |
Serum (n=5) | 78-95 | 87 |
EDTA Plasma (n=5) | 80-98 | 84 |
Heparin Plasma (n=5) | 84-92 | 89 |
- Kit Detection Method:
- Chemiluminescent, visible wavelength light
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express CXCL14.

- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express CXCL14.

- Datasheets/Manuals:
- Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.