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CXCL14 Chemi-Luminescent ELISA Kit (Human) (OKCD03339)

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Predicted Species Reactivity:
Human
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
CXCL14
Alias Symbols:
BMAC, bolekine, BRAK, Chemokine BRAK, C-X-C motif chemokine 14, Kec, KEC, KS1, MGC10687, MIP2G, MIP-2g, MIP-2G, NJAC, PSEC0212, SCYB14, Small-inducible cytokine B14, UNQ240/PRO273
NCBI Gene Id:
9547
Protein Name:
C-X-C motif chemokine 14
Description of Target:
Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.
Swissprot Id:
O95715
Protein Accession #:
NP_004878
Nucleotide Accession #:
NM_004887
Sample Type:
Serum, plasma and other biological fluids
Sensitivity:
0.156 ng/mL
Kit Range:
0.156 - 10 ng/mL
Kit Reproducibility:
Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CXCL14. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CXCL14 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology CXCL14 Chemi-Luminescent ELISA Kit (Human) (OKCD03339) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXCL14 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXCL14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample CXCL14
Kit Component:
ComponentAmount
CXCL14 Microplate96 Wells (12 x 8 Well strips)
CXCL14 Lyophilized Standard2
100X CXCL14 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Kit Linearity:
Sample1:21:41:81:16
Serum (n=5)90-99%89-104%98-105%86-93%
EDTA Plasma (n=5)80-101%94-103%78-102%96-103%
Heparin Plasma (n=5)92-99%88-96%83-99%86-94%
Kit Recovery:
MatrixRecovery range (%)Average(%)
Serum (n=5)78-9587
EDTA Plasma (n=5)80-9884
Heparin Plasma (n=5)84-9289
Kit Detection Method:
Chemiluminescent, visible wavelength light
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CXCL14.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CXCL14.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA

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