CXCL14 Chemi-Luminescent ELISA Kit (Human) (OKCD03339)

Catalog#: OKCD03339

• Predicted Species Reactivity: Human
• Reconstitution and Storage: Store as indicated in product manual.
• Gene Symbol: CXCL14
• Alias Symbols: BMAC, bolekine, BRAK, Chemokine BRAK, C-X-C motif chemokine 14, Kec, KEC, KS1, MGC10687, MIP2G, MIP-2g, MIP-2G, NJAC, PSEC0212, SCYB14, Small-inducible cytokine B14, UNQ240/PRO273
• NCBI Gene Id: 9547
• Protein Name: C-X-C motif chemokine 14
• Description of Target: Potent chemoattractant for neutrophils, and weaker for dendritic cells. Not chemotactic for T-cells, B-cells, monocytes, natural killer cells or granulocytes. Does not inhibit proliferation of myeloid progenitors in colony formation assays.
• Swissprot Id: O95715
• Protein Accession #: NP_004878
• Nucleotide Accession #: NM_004887
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.156 ng/mL
• Kit Range: 0.156 - 10 ng/mL
• Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CXCL14. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CXCL14 using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• Kit Duration: ~ 3.5 Hours
• Kit Principle: Aviva Systems Biology CXCL14 Chemi-Luminescent ELISA Kit (Human) (OKCD03339) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXCL14 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXCL14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample CXCL14
• Kit Component:

  Component	Amount
  CXCL14 Microplate	96 Wells (12 x 8 Well strips)
  CXCL14 Lyophilized Standard	2
  100X CXCL14 Biotinylated Detector Anitbody	1 x 120 uL
  100X Avidin-HRP Conjugate	1 x 120 uL
  Standard Diluent	1 x 20 mL
  Detector Antibody Diluent	1 x 12 mL
  Conjugate Diluent	1 x 12 mL
  30X Wash Buffer	1 x 20 mL
  100X Luminol Substrate	1 x 2 mL
  Substrate Diluent	1 x 20 mL

• Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	90-99%	89-104%	98-105%	86-93%
  EDTA Plasma (n=5)	80-101%	94-103%	78-102%	96-103%
  Heparin Plasma (n=5)	92-99%	88-96%	83-99%	86-94%

• Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-95	87
  EDTA Plasma (n=5)	80-98	84
  Heparin Plasma (n=5)	84-92	89

• Kit Detection Method: Chemiluminescent, visible wavelength light
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express CXCL14.
• Datasheets/Manuals: Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Protocol:

Tips Information: