CXCL13 ELISA Kit (Mouse) (OKCD07516)

Catalog No: OKCD07516

Size:96WELLS

CXCL13 ELISA Kit (Mouse) (OKCD07516) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Lymphocyte Chemoattractant (BLC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Lymphocyte Chemoattractant (BLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Lymphocyte Chemoattractant (BLC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of B-Lymphocyte Chemoattractant (BLC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Lymphocyte Chemoattractant (BLC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Lymphocyte Chemoattractant (BLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CXCL13 Microplate: 96 Wells (12 x 8 Well strips)
  • CXCL13 Lyophilized Standard: 2
  • 100X CXCL13 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 5.9pg/mL

Target Info

• Gene Symbol: Cxcl13
• Gene Full Name: chemokine (C-X-C motif) ligand 13
• Alias Symbols: 4631412M08Rik;ANG;Angie;ANGIE2;B lymphocyte chemoattractant;BCA-;BCA-1;BL;BLC;BLR1L;CXC chemokine BLC;C-X-C motif chemokine 13;Scyb1;Scyb13;small inducible cytokine subfamily B (Cys-X-Cys), member 13;small-inducible cytokine B13.
• NCBI Gene Id: 55985
• Protein Name: C-X-C motif chemokine 13
• Description of Target: Strongly chemotactic for B-lymphocytes, weakly for spleen monocytes and macrophages but no chemotactic activity for granulocytes. Binds to BLR1/CXCR5. May play a role in directing the migration of B-lymphocytes to follicles in secondary lymphoid organs.
• Uniprot ID: O55038
• Protein Accession #: NP_061354.1
• Nucleotide Accession #: NM_018866.2