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Catalog No: OKCD03318
Size:96WELLS
Price: $837.00
SKU
OKCD03318
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CXCL13 Chemi-Luminescent ELISA Kit (Rat) (OKCD03318)

Datasheets/ManualsPrintable datasheet for CXCL13 Chemi-Luminescent ELISA Kit (Rat) (OKCD03318)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)95-103%80-101%92-105%78-92%
EDTA Plasma (n=5)79-101%85-102%92-99%81-98%
Heparin Plasma (n=5)94-101%79-97%80-102%93-104%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to B-Lymphocyte Chemoattractant (BLC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to B-Lymphocyte Chemoattractant (BLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the B-Lymphocyte Chemoattractant (BLC) level in the sample or standard.
ELISA Kit Range1.37-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)93-10197
EDTA Plasma (n=5)93-10299
Heparin Plasma (n=5)78-10182
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Lymphocyte Chemoattractant (BLC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Lymphocyte Chemoattractant (BLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Cxcl13 Microplate96 Wells (12 x 8 Well strips)
Cxcl13 Lyophilized Standard2
100X Cxcl13 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity15.63 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCxcl13
Gene Full NameC-X-C motif chemokine ligand 13
Alias Symbolschemokine (C-X-C motif) ligand 13;C-X-C motif chemokine 13.
NCBI Gene Id498335
Protein NameChemokine (C-X-C motif) ligand 13
Description of TargetChemokine (C-X-C motif) ligand 13
Uniprot IDQ5I0J6
Protein Accession #NP_001017496
Nucleotide Accession #NM_001017496
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