CXCL13 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03320)

Catalog No: OKCD03320

Size:96WELLS

Mouse CXCL13 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	99-105%	93-105%	81-97%	86-98%
  EDTA Plasma (n=5)	82-91%	78-103%	86-94%	89-98%
  Heparin Plasma (n=5)	86-105%	89-98%	95-102%	91-99%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to B-Lymphocyte Chemoattractant (BLC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to B-Lymphocyte Chemoattractant (BLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the B-Lymphocyte Chemoattractant (BLC) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	80-91	80
  EDTA Plasma (n=5)	80-102	99
  Heparin Plasma (n=5)	78-101	82

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level B-Lymphocyte Chemoattractant (BLC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level B-Lymphocyte Chemoattractant (BLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Cxcl13 Microplate: 96 Wells (12 x 8 Well strips)
  • Cxcl13 Lyophilized Standard: 2
  • 100X Cxcl13 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 15.63 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cxcl13
• Gene Full Name: chemokine (C-X-C motif) ligand 13
• Alias Symbols: 4631412M08Rik;ANG;Angie;ANGIE2;B lymphocyte chemoattractant;BCA-;BCA-1;BL;BLC;BLR1L;CXC chemokine BLC;C-X-C motif chemokine 13;Scyb1;Scyb13;small inducible cytokine subfamily B (Cys-X-Cys), member 13;small-inducible cytokine B13.
• NCBI Gene Id: 55985
• Protein Name: C-X-C motif chemokine 13
• Description of Target: Strongly chemotactic for B-lymphocytes, weakly for spleen monocytes and macrophages but no chemotactic activity for granulocytes. Binds to BLR1/CXCR5. May play a role in directing the migration of B-lymphocytes to follicles in secondary lymphoid organs.
• Uniprot ID: O55038
• Protein Accession #: NP_061354
• Nucleotide Accession #: NM_018866