- NCBI Gene Id:
- Protein Name:
- Stromal cell-derived factor 1
- Alias Symbols:
- IRH, PBSF, SDF1, TLSF, SDF1A, SDF1B, TPAR1, SCYB12, CXCL12
- Replacement Item:
- This antibody may replace item sc-133989 from Santa Cruz Biotechnology.
- Description of Target:
- RABBIT ANTI HUMAN SDF-1 ALPHA
- Protein Size (# AA):
- ELISA, WB
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express CXCL12.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express CXCL12.
- The immunogen for anti-CXCL12 antibody: recombinant human SDF-1 alpha.
- Predicted Species Reactivity:
- Predicted Homology Based on Immunogen Sequence:
- Product Format:
- Purified IgG - lyophilised
- Reconstitution and Storage:
- Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Storage: Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Printable datasheet for anti-CXCL12 antibody - OASA08153
- SDF-1 ALPHA
- Polyclonal IgG
- Application Info:
- ELISA: 0.5ug/ml
Western Blotting: 0.1 - 0.2ug/ml
Functional Assays: 2.0 - 4.0ug/ml
- Preservative Stabilisers: None present
Antiserum Preparation: Antisera to human SDF-1 alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Approx Protein Conc: IgG concentration 1.0mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
- Target Reference:
- 1. Nagasawa, T. et al. (1998) A novel CXC chemokine PBSF/SDF-1 and its receptor CXCR4: their functions in development, hematopoiesis and HIV infection. Seminars in Immunology 10: 179-185.
2. Stumm, R. and Hollt, V. (2007) CXC chemokine receptor 4 regulates neuronal migration and axonal pathfinding in the developing nervous system: implications for neuronal regeneration in the adult brain. J. Mol. Endocrinol. 38: 377-382.
3. Kucia, M. et al. (2005) Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: Pivotal role of the SDF-1-CXCR4 axis. Stem Cells. 23: 879-894.