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CXCL12 Antibody (OASA08153)

0.1 mg
$609.00
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Gene Symbol:
CXCL12
NCBI Gene Id:
6387
Protein Name:
Stromal cell-derived factor 1
Swissprot Id:
P48061
Protein Accession #:
NP_000600.1
Alias Symbols:
IRH, PBSF, SDF1, TLSF, SDF1A, SDF1B, TPAR1, SCYB12, CXCL12
Replacement Item:
This antibody may replace item sc-133989 from Santa Cruz Biotechnology.
Description of Target:
RABBIT ANTI HUMAN SDF-1 ALPHA
Protein Size (# AA):
93
Host:
Rabbit
Clonality:
Polyclonal
Application:
ELISA, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CXCL12.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CXCL12.
Immunogen:
The immunogen for anti-CXCL12 antibody: recombinant human SDF-1 alpha.
Predicted Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified IgG - lyophilised
Reconstitution and Storage:
Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Storage: Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-CXCL12 antibody - OASA08153
Specificity:
SDF-1 ALPHA
Isotype:
Polyclonal IgG
Application Info:
ELISA:    0.5ug/ml
Western Blotting:    0.1 - 0.2ug/ml
Functional Assays:    2.0 - 4.0ug/ml
:::
Preservative Stabilisers: None present
Antiserum Preparation: Antisera to human SDF-1 alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
:::
Approx Protein Conc: IgG concentration 1.0mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Nagasawa, T. et al. (1998) A novel CXC chemokine PBSF/SDF-1 and its receptor CXCR4: their functions in development, hematopoiesis and HIV infection. Seminars in Immunology 10: 179-185.
2. Stumm, R. and Hollt, V. (2007) CXC chemokine receptor 4 regulates neuronal migration and axonal pathfinding in the developing nervous system: implications for neuronal regeneration in the adult brain. J. Mol. Endocrinol. 38: 377-382.
3. Kucia, M. et al. (2005) Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: Pivotal role of the SDF-1-CXCR4 axis. Stem Cells. 23: 879-894.

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