CXCL11 ELISA Kit (Rat) (OKCD00782)

Catalog No: OKCD00782

Size:96WELLS

Rat Cxcl11 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	84-94%	99-105%	96-105%	78-92%
  EDTA plasma(n=5)	98-105%	93-105%	97-104%	81-97%
  heparin plasma(n=5)	90-98%	80-92%	82-101%	99-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant (ITaC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant (ITaC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Inducible T-Cell Alpha Chemoattractant (ITaC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	85-101	89
  EDTA plasma(n=5)	78-92	81
  heparin plasma(n=5)	90-104	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Cxcl11 Microplate: 96 Wells (12 x 8 Well Strips)
  • Cxcl11 Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated Cxcl11 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 19.83 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interferon Inducible T-Cell Alpha Chemoattractant (ITaC). No significant cross-reactivity or interference between Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Cxcl11
• Gene Full Name: C-X-C motif chemokine ligand 11
• Alias Symbols: chemokine (C-X-C motif) ligand 11;C-X-C motif chemokine 11;SCYB11.
• NCBI Gene Id: 305236
• Protein Name: C-X-C motif chemokine
• Description of Target: C-X-C motif chemokine
• Uniprot ID: Q7TNL0
• Protein Accession #: NP_891997
• Nucleotide Accession #: NM_182952.2
• Protein Size (# AA): 100