CXCL11 Chemi-Luminescent ELISA Kit (Rat) (OKCD03694)

Catalog No: OKCD03694

Size:96WELLS

Rat CXCL11 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	83-101%	94-105%	97-105%	89-104%
  EDTA Plasma (n=5)	78-92%	92-101%	93-101%	95-102%
  Heparin Plasma (n=5)	79-102%	90-103%	84-101%	79-92%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant (ITaC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant (ITaC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	90-97	93
  EDTA Plasma (n=5)	78-99	89
  Heparin Plasma (n=5)	81-101	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Cxcl11 Microplate: 96 Wells (12 x 8 Well strips)
  • Cxcl11 Lyophilized Standard: 2
  • 100X Cxcl11 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 31.25 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cxcl11
• Gene Full Name: C-X-C motif chemokine ligand 11
• Alias Symbols: chemokine (C-X-C motif) ligand 11;C-X-C motif chemokine 11;SCYB11.
• NCBI Gene Id: 305236
• Protein Name: C-X-C motif chemokine
• Description of Target: C-X-C motif chemokine
• Uniprot ID: Q7TNL0
• Protein Accession #: NP_891997
• Nucleotide Accession #: NM_182952