Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCD03933
Size:96WELLS
Price: $925.00
SKU
OKCD03933
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

CXCL11 Chemi-Luminescent ELISA Kit (Bovine) (OKCD03933)

Datasheets/ManualsPrintable datasheet for CXCL11 Chemi-Luminescent ELISA Kit (Bovine) (OKCD03933)
Product Info
Predicted Species ReactivityBos taurus|Bovine
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)86-101%94-103%97-104%91-98%
EDTA Plasma (n=5)92-101%79-98%80-95%83-94%
Heparin Plasma (n=5)79-101%90-104%95-105%87-101%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant (ITaC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant (ITaC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) level in the sample or standard.
ELISA Kit Range4.12-3,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)81-9994
EDTA Plasma (n=5)92-10197
Heparin Plasma (n=5)89-9692
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Cxcl11 Microplate96 Wells (12 x 8 Well strips)
Cxcl11 Lyophilized Standard2
100X Cxcl11 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity46.88 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCXCL11
Gene Full NameC-X-C motif chemokine ligand 11
Alias SymbolsC-X-C motif chemokine 11;I-TAC;small-inducible cytokine B11.
NCBI Gene Id516104
Protein NameC-X-C motif chemokine 11
Description of TargetChemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses (By similarity).
Uniprot IDA9QWQ1
Protein Accession #NP_001106644
Nucleotide Accession #NM_001113173
Write Your Own Review
You're reviewing:CXCL11 Chemi-Luminescent ELISA Kit (Bovine) (OKCD03933)
Your Rating
We found other products you might like!