Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any help please email info@avivasysbio.com

Catalog No: OKEH04522
Size:96T
Price: $425.00
SKU
OKEH04522
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Cxcl10 ELISA Kit (Mouse) (OKEH04522) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cxcl10 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Cxcl10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cxcl10 captured in well.
ELISA Kit Range62.5-4000pg/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-Cxcl10 Microplate96 Wells (12 x 8 Well Strips)
Cxcl10 Lyophilized Standard2
Sample Diluent1 x 20 mL
Cxcl10 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity31.29 pg/mL
SpecificityNatural and recombinant Mouse C-X-C motif chemokine 10
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCxcl10
Gene Full Namechemokine (C-X-C motif) ligand 10
Alias Symbols10 kDa interferon gamma-induced protein;C7;CRG-;CRG-2;C-X-C motif chemokine 10;gamma-IP10;gIP-;gIP-10;Ifi;Ifi10;IN;INP10;interferon activated gene 10;interferon-gamma induced protein CRG-2;IP;IP-;IP10;IP-10;mob-;mob-1;Scyb1;Scyb10;small inducible cytokine B subfamily (Cys-X-Cys), member 10;small-inducible cytokine B10.
NCBI Gene Id15945
Protein NameP17515
Description of TargetC-X-C motif chemokine 10
Uniprot IDhttps://www.uniprot.org/uniprot/P17515
Protein Accession #NP_067249.1
Nucleotide Accession #NM_021274.2
Protein Size (# AA)98
Molecular Weight11 KDa
Write Your Own Review
You're reviewing:Cxcl10 ELISA Kit (Mouse) (OKEH04522)
Your Rating
We found other products you might like!