Catalog No: OKCD00162 (Formerly GWB-KBBOE4)
Size:96WELLS
Price: $500.00
SKU
OKCD00162
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Datasheets/ManualsPrintable datasheet for CXCL10 ELISA Kit (Mouse) (OKCD00162)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)99-105%83-93%80-102%95-103%
EDTA plasma(n=5)81-90%78-94%78-89%80-93%
heparin plasma(n=5)82-93%87-98%95-105%90-97%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Gamma Induced Protein 10kDa (IP10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Gamma Induced Protein 10kDa (IP10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Gamma Induced Protein 10kDa (IP10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interferon Gamma Induced Protein 10kDa (IP10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)78-10292
EDTA plasma(n=5)82-9387
heparin plasma(n=5)84-9591
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Gamma Induced Protein 10kDa (IP10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Gamma Induced Protein 10kDa (IP10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Cxcl10 Microplate96 Wells (12 x 8 Well Strips)
Cxcl10 Lyophilized Standard2 x 1 ng
100X Biotinylated Cxcl10 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 6.7 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interferon Gamma Induced Protein 10kDa (IP10). No significant cross-reactivity or interference between Interferon Gamma Induced Protein 10kDa (IP10) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCxcl10
Gene Full Namechemokine (C-X-C motif) ligand 10
Alias Symbols10 kDa interferon gamma-induced protein;C7;CRG-;CRG-2;C-X-C motif chemokine 10;gamma-IP10;gIP-;gIP-10;Ifi;Ifi10;IN;INP10;interferon activated gene 10;interferon-gamma induced protein CRG-2;IP;IP-;IP10;IP-10;mob-;mob-1;Scyb1;Scyb10;small inducible cytokine B subfamily (Cys-X-Cys), member 10;small-inducible cytokine B10.
NCBI Gene Id15945
Protein NameC-X-C motif chemokine
Description of TargetC-X-C motif chemokine
Uniprot IDQ548V9
Protein Accession #NP_067249
Nucleotide Accession #NM_021274.2
Protein Size (# AA)98
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